PG Department of Microbiology undertook a comprehensive water quality assessment program on 22/03/2022 to evaluate the microbial pollution levels in local water sources. The primary method employed was the Most Probable Number (MPN) technique, a widely accepted method for quantifying the concentration of coliform bacteria in water samples. Water samples were collected from various sources, including wells, municipal water supplies. The MPN method was used to estimate the number of coliform bacteria present in each water sample. The MPN analysis revealed varying levels of coliform contamination in the water samples. Some sources exhibited low coliform counts, indicating good water quality, while others had significantly higher counts, suggesting contamination with faecal matter or other pollutants and conveyed the customer about the risk and guide them to decontaminate the water. The results highlight the importance of monitoring water quality, especially in regions with limited access to clean water. High coliform counts in certain samples indicate potential health risks associated with waterborne diseases. It emphasizes the need for proper sanitation practices and the treatment of water sources to ensure safe drinking water for communities. The water quality assessment program conducted by the PG Department of Microbiology underscored the importance of regular monitoring and intervention in ensuring safe and clean water sources for communities. The MPN technique proved to be a valuable tool in assessing microbial contamination levels. Continued efforts in water quality assessment and improvement are essential to safeguard public health and the environment.